BRAFV600E mutation and DNA ploidy determination in fine needle aspiration biopsy of papillary thyroid microcarcinoma in a Chinese population

نویسندگان

  • Liangyan Zhang
  • Shengli Yan
  • Guoliang Sui
  • Yangang Wang
  • Yubo Ren
  • Chuanhong Li
چکیده

This study aimed to evaluate the diagnostic value of BRAFV600E mutation and DNA ploidy determination for Papillary Thyroid Micro-Carcinoma (PTMC) through fine-needle aspiration. Clinical data, including histology, patient population statistics and clinical results were gathered. Patients’ thyroid-micro-lesions were made into FNA-stained smears, DNA was extracted from the smears for different cytological diagnoses. BRAFV600E mutation was detected by Amplification Refractory Mutation System (ARMS). DNA ploidy determination of PTMC was detected by DNA quantitative analyser. All surgical cases were collected for histopathological diagnosis. BRAFV600E mutation was identified in 168 of 180 (93.3%) of PTMC, 0/9 of thyroid follicular carcinomer and 0/1389 of benign thyroid nodules. 127/180 (70.5%) patients got a definitive diagnosis of PTMC when only use cytology. 177/180 (98.3%) patients got a definitive diagnosis of PTMC by a combination of cytology and BRAFV600E mutation analysis (p<0.05). DNA ploidy determination showed no abnormal on papillary thyroid microcarcinoma. BRAFV600E mutation could be only detected in Papillary Thyroid Carcinoma (PTC) patients and is the most common oncogenic alteration in PTC patients. Thus BRAFV600E mutation is a genetic marker of PTMC. BRAFV600E mutation analysis via fine-needle aspiration improved 27.8% of the diagnosis rate of PTMC when combined with cytology.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Association of BRAFV600E mutation with clinicopathological features of papillary thyroid carcinoma: a study on a Chinese population.

BACKGROUND The new finding of the heterogeneous distribution of BRAF(V600E) mutation in primary papillary thyroid carcinoma suggested the percentage of BRAF(V600E) alleles should be taken into consideration when evaluating its association with clinicopathological features of papillary thyroid carcinoma. The aim of this study was to detect both the presence and the percentage of BRAF(V600E) alle...

متن کامل

Preoperative BRAF Mutation is Predictive of Occult Contralateral Carcinoma in Patients with Unilateral Papillary Thyroid Microcarcinoma

Thyroid cancer is the most common endocrine malignancy, accounting for 1% of all malignant tumors in the USA (Sherman, 2003) and 5.9% in one district of Wenzhou (a coastal city in Southeast China, with a incidence of 21/100,000) (Zheng and Zhang, 2007). The incidence of papillary thyroid carcinoma, which is the most common histologic type of thyroid malignancy, is increasing worldwide (Davies a...

متن کامل

Impact of Molecular Testing in the Diagnosis of Thyroid Fine Needle Aspiration Cytology: Data from Mainland China

BACKGROUND The molecular work-up of thyroid nodules from fine needle aspiration samples has given clinicians a new level of diagnostic information. The aim of the present study was to evaluate the utility of molecular analysis in thyroid fine needle aspiration samples from a Chinese population. METHODS Specimens were collected from thyroid nodules by fine needle aspiration. Cytology diagnosis...

متن کامل

Successful sonography-guided fine-needle aspiration biopsy of a 1-millimeter-diameter papillary thyroid microcarcinoma.

A PTMC is defined as a thyroid papillary cancer that measures < or =10 mm in the greatest diameter. The detection rate for a PTMC has increased because of the worldwide use of high-resolution sonography and US-FNAB. We report a case of a 1-mm-diameter PTMC confirmed at thyroidectomy for which the cytologic diagnosis had an important role in deciding the extent of surgery (ie, either a total thy...

متن کامل

Diagnostic value of ultrasound-guided fine needle aspiration biopsy in malignant thyroid nodules: utility for micronodules.

BACKGROUND The diagnostic approach to thyroid nodules involves ultrasound-guided fine needle aspiration biopsy (US-FNAB).We especially aimed to evaluate the contribution and the place of US-FNAB in preoperative evaluation of the malignant cases and draw attention to discordant cases diagnosed with papillary thyroid microcarcinoma (PTMC). MATERIALS AND METHODS A total of 276 cases were retrosp...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2017